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Genotype offspring from heterozygous matings
| Age
|
+/+
|
+/−
|
−/−
|
−/−(%)
|
|---|---|---|---|---|
| E11.5 | 9 | 18 | 9 | 25 |
| E12.5 | 15 | 25 | 10 | 20 |
| E13.5 | 20 | 40 | 6 | 9 |
| E14.5 | 16 | 29 | 3 | 6 |
| E15.5 | 21 | 41 | 2 | 3 |
| E16.5 | 11 | 25 | 0 | 0 |
| E18.5 | 16 | 36 | 0 | 0 |
| P1 | 72 | 149 | 0 | 0 |
Genotyping was performed by PCR analysis of yolk sac or tail DNA using primers diagnostic for wild-type and mutant alleles. Noon of the day on which a vaginal plug was detected was defined as E0.5 of gestation. (P1) postnatal day 1.
