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. 2011 Sep 15;22(18):3331–3343. doi: 10.1091/mbc.E10-10-0853

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© 2011 Topf and Chiquet-Ehrismann. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Epidermal defects correlate with defects in body wall muscles in arrested ten-1 phy-1 embryos. (A) TEN-1 is expressed in dorsal epidermal cells during embryogenesis. (B, C) Confocal images of embryos expressing mCherry under the muscle-specific myo-3 promoter. (B) Double-mutant embryo arrested at the 2.5-fold stage. Muscle strands are disrupted (arrowhead) and detached from the epidermal cells (arrow). (C) Wild-type embryos at the threefold stage show continuous strands of body wall muscles from head to tail. (D–F) Three-dimensional representation combining the epidermal (green) and muscle (red) markers in an arrested ten-1 phy-1 embryo. (D) Expression of pten-1b::gfp. The major epidermal defect is indicated by an asterisk. (E) Expression of pmyo-3::mCherry. The disrupted muscle strand is indicated by an arrowhead. (F) Merged image showing that the epidermal and muscle defects are in close proximity. Scale bar, 10 μm.