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Knockdown of phy-2 in phy-1(ok162) mutants increases intracellular aggregation of collagen IV. Confocal images of phy-1(ok162) mutant embryos on control RNAi (A, C) or phy-2 RNAi (B, D) stained with anti–LET-2 or anti–EMB-9 antibodies. (A, C) Control embryos at the threefold stage show few collagen IV aggregates. Collagen IV protein localizes to BMs. (B, D) Elongation arrested embryos show an increase in collagen IV aggregates, and the muscle BM appears disorganized. Scale bar, 10 μm.
