Table 1.
Strains and plasmids used in this work
| Bacterium or plasmid
|
Genotype or relevant characteristic(s)
|
Source
|
|---|---|---|
| E. coli strains | ||
| W3110 | F− IN[rrnD–rrnE]1 | F.C. Neidhardt (University of Michigan, Ann Arbor) |
| DHB4 | F′ lac–pro lacIQ/[ara–leu]7697 araD139 lacX74 galE galK rpsL phoR (phoA) PvuII malF3 thi | W. Prinz (Harvard University, Boston, MA) |
| WP551 | DHB4 + pAID135 | W. Prinz |
| WP552 | WP551 trxB::Km | W. Prinz |
| WP841 | WP551 gor522 mini-Tn10Tc | W. Prinz |
| TN5001 | WP551 rpoS::Km | this work |
| TN5002 | WP551 oxyR::Km | this work |
| SL396 | lacZ-350 galK2 galT22 rpsL179 λpF13 (PgroE::lacZ)F′ | C. Gross (UCSF Medical Center) |
| SD18 | SL396 Φ(sodA′–′lacZ)49 Φ(sodB–kan)Δ2 | this work |
| SD20 | SL396 katE::Tn10 katG::Tn10 | this work |
| SD22 | SD20 Φ(sodA′–′lacZ)49 Φ(sodB–kan)Δ2 | this work |
| SD16 | SL396 + pDT1-19 | this work |
| MG1655 | F− | D. Touati (University of Paris, France) |
| QC2413 | MG1655 oxyR::Km | D. Touati |
| QC2410 | MG1655 rpoS::Tn10 | D. Touati |
| Plasmids | ||
| pAID135 | Δ2-22AP under Ptac control | W. Prinz |
| pDT1–19 | pBR322 carrying the lacI gene and the SodA gene under Ptac control | D. Touati |