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. 2011 Sep 15;22(18):3355–3365. doi: 10.1091/mbc.E11-02-0165

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© 2011 Nie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — CaD-MO disrupted CNC migration in vivo. (A) GFP-labeled CNC grafts receiving control-MO or CaD-MO (10 ng) were transplanted into unlabeled host embryos at neurula stages and imaged at tailbud stages. Fluorescent and merged images are shown side by side with anterior to the left. Whereas control-MO–receiving grafts migrated laterally into separate branchial arches, CaD-MO–expressing grafts migrated less far and failed to segregate into streams. Coexpression of a low-dose CaD restored the migration of CNC transplants. (B) The embryos were binned into four categories on the basis of the migratory behaviors. Whereas 79% of control grafts migrated and segregated normally (n = 61), 18% of CaD-MO–expressing grafts failed to migrate at all. Another 67% of the grafts migrated shorter distances, over half of which did not segregate (n = 45). Coexpression of CaD (100–200 pg) rescued the segregation (83%) and migration (44%, n = 18) remarkably.