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. 2011 Sep 15;22(18):3394–3409. doi: 10.1091/mbc.E11-02-0155

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© 2011 Saw et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 11: — Down-regulation of Voa1, Voa2, and Voa1/Voa2 causes significant reductions in the amount of endogenous dopamine stored in PC12 cells. Endogenous dopamine concentrations were measured using HPLC for Voa1 knockdown and its control (A, n = 9 each), Voa2 knockdown and its control (B, n = 25 each), or Voa1/Voa2 double knockdown and its control (D, n = 15 each). Values were normalized with the total protein concentrations of the samples. Error bars, SEM. (C) Sample picture of HPLC data showing differences in dopamine amounts between control and Voa1/Vao2 double-knockdown cells. Endogenous dopamine concentrations were measured using HPLC for Voa1/Voa2 double knockdown and its control (F, n = 6 each) from purified plasma membrane–associated DCV. Of this purified sample, 2.5 μg was immunoblotted with anti–secretogranin II rabbit polyclonal antibody (E).