FIGURE 4:
Endogenous Voa1 colocalizes with syntaptotagmin-1. NGF-differentiated PC12 cells were costained with anti-Voa1 rabbit polyclonal antibody and anti–synaptotagmin-1 mouse monoclonal antibody (A, B) or with anti-Voa2 rabbit polyclonal antibody and GM130 mouse monoclonal antibody (C). For secondary staining Alexa 488–conjugated goat anti–rabbit antibody and Rhodamine Red-X–conjugated goat anti–mouse antibody were used against the appropriate primary antibodies. Right, merged pictures. Scale bar, 10 μm