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. 2011 Sep 15;22(18):3410–3419. doi: 10.1091/mbc.E11-02-0168

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© 2011 Kibanov et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Partial colocalization of Vasa and Tud in the piNG-body. (A) The piNG-body stained with anti-Vasa (green) and anti-Tud (red) antibodies, as well as the corresponding pseudo-3D image, are shown. Testes of yw flies were stained with anti-Vasa (green), anti-Tud (red), and anti-lamin (violet) antibodies; chromatin was stained with DAPI (blue). Colocalization of green and red signals yields yellow color. Scale bars, 3 μm. (B) An isosurface image for the piNG-body presented in A. In spite of the observed significant colocalization of Vasa and Tud signals, nonoverlapping zones are clearly seen.