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. 2011 Sep 15;22(18):3410–3419. doi: 10.1091/mbc.E11-02-0168

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© 2011 Kibanov et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Stellate derepression analysis. Western blots of testis extracts probed with anti-Stellate antibodies are shown. Mutations in bel (A), tud (B), and cuff (D) cause no Stellate derepression, whereas mutations in vasa [vas^EP812/vas^D1, vas^EP812/vas^PH165 (E), and vas^EP812 (F)], ago3 (F), and squ (C) lead to Stellate hyperexpression. Testes of crystal (cry) and yw flies were used as positive and negative controls, respectively. Anti-actin antibodies were used as a loading control. Anti-Vasa and anti-VIG antibodies were used for the analysis of vasa allelic combinations presented in E. Note trace Stellate derepression in the vas^EP812/vas^PD hypomorphic combination. vas^EP812 mutant presented in F expresses neither VIG nor Vasa proteins ( Vagin et al., 2004; Gracheva et al., 2009).