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. 2011 Sep 15;22(18):3478–3490. doi: 10.1091/mbc.E11-06-0482

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© 2011 Acosta et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — CDK inhibition further compromises Ndt80 induction in zip1 cells. (A) Time-course analysis of meiotic nuclear divisions. The percentage of cells containing more than two nuclei is represented. Cultures were split in two 12 h after meiotic induction (arrow) to allow premeiotic S phase to occur, and 1 μM of 1NM-PP1 (dissolved in DMSO) was added to one-half of the culture. The same amount of DMSO alone was added to the other half. (B) Western blot analysis of Cdc5 and Ndt80 production in the meiotic cultures described in A. PGK was used as a loading control. Strains are cdc28-as1 (DP430/pRS426), zip1 cdc28-as1 (DP431/pRS426), and zip1 cdc28-as1 2μ-CDC5 (DP431/pJC29).