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. 2011 Sep 15;22(18):3520–3532. doi: 10.1091/mbc.E11-04-0295

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© 2011 Promlek et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 9: — Cellular localization and activity of bZIP-Ire1. (A) The ire1Δ strain KMY1516 transformed with wild-type (WT) pRS423-IRE1-HA or its bZIP mutant version (or empty vector pRS423 [Vector]) was cultured under the indicated conditions and stained by mouse anti-HA antibody/FITC-labeled secondary antibody. (B) bZIP-Ire1-HA cells used in (A) were double-stained with rabbit anti-HA antibody/Cy5-labeled secondary antibody and rabbit anti–yeast BiP antibody/FITC-labeled secondary antibody. (C and D) The ire1Δ strain transformed with the bZIP mutant version of pRS313-IRE1 was stressed by the indicated stimuli. Total RNA samples were then analyzed by RT-PCR to evaluate splicing efficiency of the HAC1 mRNA. Error bars represent the SDs from three independent transformants.

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