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. 2011 Sep 13;6(9):e24844. doi: 10.1371/journal.pone.0024844

Figure 2. Cytotoxicity Assay.

Figure 2

Cytotoxicity of compounds. A) Fluphenazine B) trimipramine C) #23I and D) #59 were applied to N2a/22L cells for 72 hours. Cells were then transferred to 96 well plates and allowed to attach overnight. The MTS colorimetric solution was added and allowed to incubate for 2–3 hours before reading absorbance at 490 nm. Graphs are plotted as percent cell survival compared to non-treated control. Only fluphenazine at 10 µM concentration was significantly toxic; ***p<0.0001, Student's t-test, two-tailed.