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. 2011 Sep 13;6(9):e24844. doi: 10.1371/journal.pone.0024844

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Chung et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Histological sections immunostained with anti-GFAP polyclonal antibody were quantified using Bioquant NovaPrime image analysis. A,B) Histological representation of non-treated control animal at 25× and 100× magnification, respectively (scale bars corresponding to 100 and 400 µm are shown). C,D) Shows representative sections from a treatment group (#23I) at 25× and 100× magnification. E) Shows a bar graph of the immunoreactivity quantitation for all groups. Graphs were plotted as percent immunoreactivity per area. Differences were significant by One-way ANOVA; p = 0.0073.