Table 1. Parameters corresponding to the protein-peptide interactions.
| Cuticle Peptide TAEPD(pY)GALYE | ||||||
| First Binding Site | Second Binding Site | |||||
| ka ( M−1 s−1) | kd (s−1)×10−4 | KD (µM) | ka (M−1 s−1) | kd (s−1)×10−4 | KD (µM) | |
| DLAR D1D2 | 32.6±1.57 | 102±4.80 | 312.8±0.12 | 1.65±0.23×104 | 333±4.63 | 2.02±0.15 |
| DLAR D1HSS D2 | 74.7±2.41 | 65.4±2.71 | 87.5±0.73 | 2.88±0.08×104 | 540±2.17 | 1.87±0.03 |
| DLAR D1 D2HSS | 51.7±0.61 | 153±0.62 | 295.93±0.01 | 1.37±0.4 | 0.13±0.02 | 9.43±0.45 |
| DLAR D1 | 18.6±1.46 | 17.6±4.37 | 94.6±0.33 | – | – | – |
| DLAR D2 | – | – | – | 52.8±0.69 | 55.2±1.90 | 104.5±0.06 |
SPR sensograms were fitted to a single site binding model for single domain constructs and to independent two site binding model for the double domain constructs.