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. 2011 Sep 13;6(9):e24699. doi: 10.1371/journal.pone.0024699

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(A) MacConkey agar plate-based complementation screen for plant thioesterase activity using different Zmfatb alleles. Colonies with functional alleles show varying shades of white, whereas those containing empty vector (pBC) are pink. (B) Palmitic acid concentrations produced by different alleles of Zmfatb in the bacterial expression system after 36-h cultivation. Error bars represent the standard deviation for two independent clones of each allele with three replicates per clone. pBC, the empty vector; B73, Zmfatb B73 allele; By804, Zmfatb By804 allele; IB73, Zmfatb B73 allele containing the in vitro–mutagenized 11-bp deletion.