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. 2011 Sep 13;6(9):e24957. doi: 10.1371/journal.pone.0024957

Figure 1. Effect of transient expression of HCV proteins on accumulation of reactive oxygen species (ROS).

Figure 1

Transient expression of HCV core, E1, E2, NS4B, and NS5A proteins in Huh7 cells induced oxidative stress registered by ROS formation detected by fluorometry of DCF-DA-stained cells (A). Alternatively, ROS were visualized by fluorescence microscopy, as is shown for cells expressing core protein or treated with tBHQ or/and 40 µM ROS scavenger PDTC (B). Cells treated with 100 µM tBHQ (A,B) or Tm (A) are given as positive and DMSO-treated as negative controls. Error bars indicate SD. *P<0.01 versus DMSO (Tukey-Kramer test).