Skip to main content
. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Neurooncol. 2011 Mar 4;105(1):27–44. doi: 10.1007/s11060-011-0557-x

Fig. 2.

Fig. 2

L1 was a component of exosome-like vesicles released from T98G cells. T98G cells and MDA-MB-231 human breast cancer cells were analyzed for secretion of exosomes as described in “Materials and methods” section. a Exosome vesicles in T98G cell culture using TEM showed an average of ~24 nm diameter vesicles (arrows show representative exosome vesicles). b Western blotting of exosome preparations. Mature ADAM10 (~60 kDa) was present in T98G and MDA-MB-231 exosomes. Full length L1 (220 kDa) was detected in T98G cells by UJ127 and NCAML1. UJ127 also detected two bands lower than 220 kDa, which are likely to be the L1 degradation products not generated by ADAM10, since a 32 kDa L1 cleavage product was not detected by NCAML1 antibody. MDA-MB-231 cells were used as a positive control for the exosome preparation. TSG101 is an exosome marker