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. 1998 Nov 1;12(21):3419–3430. doi: 10.1101/gad.12.21.3419

Figure 2.

Figure 2

Localization of DivIVA in division mutants. (A) Western blot demonstrating the specificity of the crude anti-DivIVA antiserum. Extracts of wild-type B. subtilis (168CA; lane 1), and a divIVA repressible strain (1756) grown in the presence (lane 2) or absence (lane 3) of inducer. Lane M indicates the positions of prestained molecular mass markers (kD). (B–H) Fluorescence micrographs showing the localization of DivIVA by immunofluorescence (B,E–G) or a DivIVA–GFP fusion (C,D,H). (B) Wild-type cells (168CA); (C) FtsZ-repressed (strain 1759); (D) FtsZ-induced (strain 1759); (E) divIB mutant (1802); (F) divIC mutant (SU347); (G,H) minD mutant (strains 1901 and 1922, respectively). (I) Phase-contrast image of the cells in H. Arrows in G and H point to relatively long cells with no central band of DivIVA. Asterisks in G and H show examples of doublet or triplet bands of DivIVA.