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. 1998 Nov 15;12(22):3541–3550. doi: 10.1101/gad.12.22.3541

Figure 2.

Figure 2

(a) Transcriptional activity of mitotic extracts (lane 2) as compared with the interphase extracts (lane 1) and complemented with different GTFs and RNAPII (lanes 3–8). Titration included all GTFs without RNAPII (lanes 3,4 ), GTFs and RNAPII without TFIIH (lanes 5,6), TFIIH and RNAPII (lanes 7,8). (Arrow) The specific transcript derived from the pMLP template. (b) Silver-staining of immunoaffinity purified TFIIH (LeRoy et al. 1998) (lane 2). (Lane 1) Molecular weight markers. (c) Reconstituted basal transcription (lane 1), dependent on TFIIH (lane 2) and TBP (lane 5). Reactions were reconstituted with immunoaffinity purified TFIIH or TFIID from interphase (lanes 3,6) or mitotic (lanes 4,7) extracts. (d) Western blot of the ERCC3 and p62 subunits of immunopurified TFIIH and of eTBP (Zhou et al. 1992), used in the reconstituted transcription in c.