Figure 3.

(a) Schematic representation of the composition of core TFIIH, the ERCC2–CAK complex, and holo-TFIIH. (b) Basal transcription by the AdML promoter was reconstituted with affinity purified mitotic TFIIH (lanes 2–4), CAK/ERCC2 affinity purified from interphase extract (lane 1), or with increasing amounts of the CAK/ERCC2 complex in the presence of mitotic TFIIH (lanes 3,4). (Arrow) The specific transcript derived from the pMLP template. (c) Western blot analysis of the immunoaffinity preparation of mitotic TFIIH and interphase CAK/ERCC2 complexes.