Fig. 3.

A high-throughput transformation method for the generation of S. pennellii T-DNA lines. a Leaf explants developing multiple shoot-buds on kanamycin-free organogenic culture medium. b Absence of growth and organogenesis in non-inoculated explants sown in kanamycin containing culture medium. c–e Inoculated explants developing shoot-buds in selective conditions. Despite the great number of transformation events per inoculated explant only those sufficiently far away to one another were selected to avoid the possibility to recover plants coming from the same transformation event. f Shoot elongation process on kanamycin-containing culture medium. g Collection of T-DNA lines in the growth chamber. h, i Acclimatization and cultivation of T-DNA lines in the greenhouse