S. pennellii mutants showing sensitivity to salinity and drought. a, b Plants grown in control conditions (a) and 200 mM NaCl for 15 days (b) of one selected line from ac. PE47, named SP salt sensitive-2; WT plants were unaffected by salt treatment at this time (close-ups), whereas mutant showed necrosis in leaves spreading from the bottom to the upper part of the plant. c, d Drought sensitivity of one selected line from ac. 20164, named SP drought sensitive-1; control plants were well-watered (c) and drought plants were watered with 30% of the nutrient solution used in the control plants for 60 days (d). The hypersensitivity symptoms were manifested by the wilting and senescence of basal leaves, which spread from the basal to the upper part of the plant, whereas WT plants only showed reduced leaf area but not symptoms of senescence (close-ups). Moreover, the reporter gene expression (GUS) was increased by stress; expression in stem vascular bundles and stomata of non-stressed plants (e, f) and drought stressed plants (g, h).
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