Table 1.
Specific viral hybridization sequences of MLPA probes
| Virus | Specific hybridization sequence of the left probe (LHS) | Specific hybridization sequence of the right probe (RHS) | Size of RT or MLPA amplicons |
|---|---|---|---|
| PAM16 Toscan-1 | GCATCCATAGTGGTCCCAGAC | ACGGGCAGGGATTCTGACAGG | 104 bp |
| PAM20 Toscan-2 | CAGGGATTCTGACAGGACACGTAG | TGCCTGA ACAGTCCACGGAAC | 107 bp |
| PAM13 SFS-1 | CCCGGCAATTAACATTGATGTGAGG | TGTCATAGACTCCTCTCCAG | 107 bp |
| PAM15 SFS-2 | TGCAACCAGGCTCTTAACTTCTC | TTGCTCCCTCTGGAGACATTCTC | 105 bp |
| PAM44 SFN S-1 | GAACCCCACCCAAATCAATGACC | AATTATTCACCCTTACCCTTCCCTCCCC | 113 bp |
| PAM45 SFN S-2 | ATTCACCCTTACCCTTCCCTC | CCCACATTGGCAGTCTACTTG | 104 bp |
| PAM84 Tahyna-1 | GAGCCTAAAGGATGTTGAGCAGC | TTAAATGGGGTAGAGGCGGCC | 106 bp |
| PAM85 Tahyna-2 | GGTGCTAGAGCAGTTCAAAGAAAATG | AAGACGCAGCTCAAAGGGAGTTG | 111 bp |
| PAM78 Inkoo-1 | TTTGGAGAGTGGCAGGTGGAGATTG | TCAATAATCATTTTCCTGGAAA + C+A + G+G* | 113 bp |
| PAM75 Inkoo-2 | GAGAGTGGCAGGTGGAGATTGTC | AATAATCATTTTCCTGGAAAACAGG | 106 bp |
| PAM100 Batai-1 | CAGATGGGGAGGAGGTTTACC | CTCTCATTCTTTCCAGGGT + C+T + G | 105 bp |
| PAM102 Batai-2 | CAGATGGGGAGGAGGTTTACC | TCTCATTCTTTCCAGGGTCTG | 104 bp |
| PAM42 Uuk S-1 | ACCATCAACGTGAAGCACCGC | GGAATGGAGGCCAAGGAGATC | 104 bp |
| PAM64 Uuk S-2 | CCCTCATCCACCTCAAGCCAAC | CCACTGACGGCATATCCACACC | 106 bp |
All sequences are given in 5′ to 3′ orientation, * nucleotides marked with + are locked nucleotides