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. 1998 Nov 15;12(22):3579–3590. doi: 10.1101/gad.12.22.3579

Figure 8.

Figure 8

GATA6-modulated transactivation of the HNF4 promoter in NIH-3T3 cells. NIH-3T3 cells were transfected with 2.5 μg of the luciferase reporter plasmid (pcHNF4Pr.luc) containing the murine HNF4 promoter and varying amounts (μg) of an expression plasmid encoding GATA6 (pcDNAG6) or the control plasmid (pcDNA3). All transfection mixtures also contained 1 μg of the pMSVβgal reference plasmid. Forty-eight hours after transfection, luciferase and β-galactosidase activities were determined. Luciferase activities corrected for differences in transfection efficiencies were normalized to luciferase activity obtained following transfection of the pHNF4Pr.luc plasmid with the pcDNA3 control plasmid. The data are presented as relative luciferase activities ± s.e.m. The mean absolute light units obtained following transfection of the pHNF4Pr.luc reporter plasmid and the control plasmid pcDNA3 was 353.