Figure 8.

Intrasplenic cells expressing glucagon are not apoptotic. (a) Apoptotic nuclei in the spleen (red color) are visualized by chromogenic detection of anti-DIG antibody without treatment with DNase I. (b) Same as a but using, in addition, an antibody for the simultaneous detection of glucagon producing cells (gpc; blue color). (c) Same as a but including a DNase I digestion step prior to the incorporation of labeled nucleotide as a positive control. (d) Same as a but omitting terminal transferase as a negative control.