Figure 3.

EIN3 homodimerization. (A) EMSA of full-size EIN3 and deletion derivatives binding to the EBS. Proteins were translated in vitro alone or in pair-wise combinations. (See Fig. 2F for description of EIN3 deletion derivatives.) (B) EIN3–EIN3 interactions assayed by the yeast two-hybrid system. Yeast cells transformed with the indicated constructs in the top left plate, were grown on synthetic complete (SC) medium (+HIS) or in SC medium without histidine (−HIS) and with 50 mm 3-aminotriazole (3-AT, Sigma) to repress basal activity of the his3 reporter gene. β-Galactosidase activity of the colonies grown in −HIS medium (LacZ) was determined by the filter-lift assay. SNF4/SNF1 were used as a positive control. Colonies from two independent transformation experiments are shown (BD-EIN3a and BD-EIN3b).