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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: J Proteome Res. 2011 Jul 25;10(9):4088–4104. doi: 10.1021/pr2002726

Figure 4. Corresponding HCD and ETD spectra of O-GlcNAc modified peptides identified in the enriched HEK293T sample.

Figure 4

Figure 4

Figure 4

Figure 4

(A–B) HCD and corresponding ETD spectra of peptide FGVS(GlcNAc)SSSSGPSQTLTSTGNFK; (C–D) HCD and corresponding ETD spectra of peptide NPFS(GlcNAc)SASGGFGSTATSNTSNLFGNSGAK. Note: “-HexNAc” or “-H2O” indicates the loss of HexNAc or H2O. The HCD spectra of both peptides exhibit the signature ion pattern of HexNAc fragments at the low m/z range, and both ETD spectra sufficient c- and z- ions with HexNAc modification for site assignment.