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. 1998 Dec 15;12(24):3872–3881. doi: 10.1101/gad.12.24.3872

Figure 4.

Figure 4

Binding of the Elongin BC complex to SOCS-1 is disrupted by mutation of the SOCS-box Elongin BC binding site motif. (Lanes 1–3) Twenty-five micrograms of total cell lysate from Sf21 cells infected with the baculovirus vectors encoding HPC4-tagged Elongin B, HSV-tagged Elongin C, and the indicated wild-type or mutant T7-tagged SOCS-1 proteins was immunoblotted with anti-T7, anti-HPC4, or anti-HSV antibodies. (Lanes 4–12) Three hundred micrograms of total cell lysate protein from baculovirus-infected Sf21 cells was immunopreciptiated with antibodies specific for T7 epitope (IP: T7-SOCS-1), HPC4 epitope (IP: HPC4-EloB), or HSV epitope (IP: HSV-EloC) and then immunoblotted with anti-T7, anti-HPC4, or anti-HSV antibodies. (H,L) Immunoglobulin heavy and light chains, respectively.