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. 1998 Dec 15;12(24):3821–3830. doi: 10.1101/gad.12.24.3821

Figure 2.

Figure 2

Southern hybridization analysis of rDNA copy numbers. The RPA135 gene was introduced by transformation into strains NOY408-1a (rpa135), NOY408-1af (rpa135 fob1), and NOY408-1af derivatives carrying FOB1 on a plasmid or control vector plasmid as indicated. DNA samples were prepared at 44 (lanes 2,6,9,12), 80 (lanes 3,7,10,13), and 116 generations (lanes 4,8,11,14) after introduction of RPA135. NOY408-1a, and NOY408-1af, which did not receive RPA135 (lanes 1,5, respectively), were also analyzed together with control strains NOY408 (lane 15) and NOY408-1b (lane 16). In addition to rDNA, a single copy gene, MCM2, was analyzed as a reference.