Table 1.
Yeast strains and plasmids
| Designation
|
Genotypes and comments
|
Source
|
|---|---|---|
| Strain | ||
| NOY408 | MATa/MATα ade2-1/ade2-1 ura3-1/ura3-1 his3-11/his3-11 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 rpa135Δ∷LEU2/RPA135 pNOY102 | Nogi et al. (1991) |
| NOY408-f | same as NOY408 except for fob1Δ∷HIS3/fob1Δ∷HIS3 | |
| NOY408-1a | MATα ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100 rpa135Δ∷LEU2 pNOY102 | Nogi et al. (1991) |
| NOY408-2a | same as NOY408-1a; freshly constructed from NOY408 | |
| NOY408-1b | MATa ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100 pNOY102 | Nogi et al. (1991) |
| NOY408-1af | same as NOY408-1a except for fob1Δ∷HIS3 | |
| NOY408-2af | the same genotype as NOY408-1af, but constructed from NOY408-f by tetrad dissection | |
| Plasmid | ||
| pNOY102 | multicopy plasmid carrying GAL7–35S rDNA, 2μ, URA3 | Nogi et al. (1991) |
| pNOY117 | CEN6, ARSH4, TRP1, RPA135 | |
| YEplac195 | multicopy plasmid vector, 2μ, URA3 | Gietz and Sugino (1988) |
| YEp–FOB1 | YEplac195 carrying FOB1 | |