Figure 2.

Knock-out of Kal7 specifically decreases surface localization of NR2B subunit. A, Exposure of cortical slices kept at 4°C to BS³ cross-links surface receptors, producing high molecular weight bands that are not observed in non-cross-linked control (Ctrl) slices; brackets indicate Surface and Intracellular receptors. As expected, an intracellular protein, tubulin, was not cross-linked. B–D, Representative Western blots of cross-linked samples from Wt and Kal7^KO slices blotted for NR2B (B), NR2A (C), and GluR1 (D). E, Surface and Intracellular receptor levels were quantified for each sample, and the Surface/Intracellular ratio for Wt slices was normalized to 100%. Kal7^KO animals show a decrease in cell surface expression of NR2B, while surface levels of NMDA receptor subunit NR2A and the AMPA receptor subunit GluR1 were unchanged. (**p = 0.018 as calculated by t test; N = 9–10 slices/genotype, 3 animals/group).