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. 1998 Dec 15;12(24):3882–3888. doi: 10.1101/gad.12.24.3882

Figure 4.

Figure 4

Determination of the molecular mass of purified cyclin A/Cdk2 by sedimentation equilibrium centrifugation. The apparent molecular mass of the cyclin A/Cdk2 kinase complex was determined as 86 ± 2 daltons. (Bottom) The raw concentration data determined by measuring the absorbance at 235 nm (cyclin A/Cdk2). The solid line drawn through the data points was obtained by fitting the fringe displacement vs. radial position to a single-species model. (Top) The residual differences between the experimental data and the fitted data for each point. The purified proteins used for analytical ultracentrifugation are shown in Fig. 6. Sedimentation equilibrium analysis was performed at 4°C and a rotor speed of 10,000 rpm.

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