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. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Chem Biol. 2011 Aug 26;18(8):1021–1031. doi: 10.1016/j.chembiol.2011.07.015

Figure 3.

Figure 3

FT-MS spectra of acyl-pantetheine ejection ions. (A) Overview of PPant ejection assay. Tandem-MS results in characteristic ejection ions bearing polyketide intermediates, which are subjected to further tandem-MS analysis to yield diagnostic fragments. In-source fragmentation of the ACP simultaneously yields multiple pantetheine ejection ions bearing different polyketide intermediates. Results from the following reactions are presented: (B) wild-type fdm PKS; and (C) fdm PKS harboring the I134F mutation in the CLF. The peak labeled (*) is a prominent, unrelated ion. Additional information for each intermediate identified by PPant ejection assay is provided in Figs S1-S7.