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. 2011 May 1;6(5):671–679. doi: 10.4161/psb.6.5.14704

Figure 2.

Figure 2

Low levels of NAE and ABA synergistically inhibit seedling growth and modulate transcript levels of ABI3 and ABI3 responsive genes. (A) Representative images of 10-day-old seedlings grown in a combination of NAE 12:0 and (+) ABA exhibit the tolerance of the FAAH overexpressor and the sensitivity of the FAAH knockout. (B) Agarose gel analysis of RT-PCR using gene specific primers to analyze gene expression in 10-day-old seedlings (treated with 20 µM NAE, 0.1 µM ABA or NAE + ABA). ABI3 was amplified for 40 cycles, AtHVA22B and RD29B for 22 cycles and 18s for 25 cycles.