Figure 1.

Transport of P to maize plants by P. indica was carried out in bi-compartment Petri dish culture system.¹⁰ (A) Radioactivity incorporated in plant was demonstrated by autoradiography. Radioactivity counts intensities are shown in false color code (vertical bar, low to high). (i) Whole maize plant before autoradiography. (ii) False-color autoradiograph of the maize plant obtained after 3 h of exposure of the maize plant. (iii) Microscopic view of a sample of plant root before autoradiography. (a) Maize plant colonized with P. indica grown at 10 µM P concentration referred as P-deprived condition (LP-C). (b) Non-colonized maize plant grown at 10 µM P concentration, (c) Maize plant colonized with P. indica grown at 10 mM P concentration referred as P-rich condition (HP-C). (d) Non-colonized maize plant grown at 10 mM P concentration. Transport assay was done at 100 mM with 1 mM of P³² (specific activity 200 mCi/mmol). (B) Amount of ³²P transferred into the maize plant components by P. indica. Bar labeled with the (*) represents significance as compared with the (HP-C) (p < 0.05). (C) PiPT gene expression analysis during colonization at P-deprived and P-rich condition. RT-PCR analysis was performed to check the PiPT gene expression in P. indica grown at P-deprived and P-rich condition. Lane 1 shows amplified PiPT DNA fragment (1.6 kb) from P. indica grown at P-deprived condition (10 mM). Lane 2, no expression of PiPT was observed when P. indica was grown at P-rich condition (10 mM). tef gene (220 bp) of P. indica was used as a loading control. A color version of this image is available at www.landesbioscience.com/journals/psb/article/15106/.