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MCP-1 ELISA analysis of supernatants of U373 cells. Supernatants of cultured U373 cells treated as indicated were analyzed with ELISA (Chemicon). Data are shown as mean + SEM of four replicates. Detection limit was 15 pg/ml human MCP-1. As compared with untreated cells (control) TNF stimulation resulted in high amounts of secreted MCP-1. PDTC blocks this induction of MCP-1 to a level below the detection limit of the assay (<15 pg/ml).
