Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jul 5;105(3):428–437. doi: 10.1038/bjc.2011.242

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2011 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Downregulation of HRF increased Mcl-1 ubiquitination. (A) (Left panel) Cells treated with CHX (200 μg ml^–1) for various time points (0–16 h) and 100 μg of cell lysates were subjected to immunoblot analysis using anti-Puma antibody. (Right panel) Cells treated with CHX for various time points (0–16 h) were immunoprecipitated for Puma and immunoblotted with anti- Mcl-1 antibody. (B) MDA-MB-468 cells treated with scrambled (Ran siRNA) or HRF siRNA was immunoblotted for Puma and GAPDH (left panel), or immunoprecipitated with ubiquitin (Ub) antibody and blotted for Mcl-1 (right panel). (C) (Left panel) Cells treated with CHX (200 μg ml^–1) for various time points (0–16 h) and 100 μg of cell lysates were subjected to immunoblot analysis using anti-HRF antibody. (Middle panel) MDA-MB-231 cells treated with scrambled (Ran siRNA) or HRF siRNA and immunoblotted using anti-HRF antibody. (Right panel) Cells treated with HRF siRNA (HR si) or with HRFsi+CHX (200 μg ml^–1) were immunoprecipitated with Mcl-1 antibody and immunoblotted using anti-Ub antibody.