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. 2011 Jul 5;105(3):407–412. doi: 10.1038/bjc.2011.246

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Copyright © 2011 Cancer Research UK

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Effects of an antibody to IL-6 and a JAK inhibitor on cell signalling and survival in gastric cancer cell lines. (A) The indicated cell lines were cultured overnight in medium containing 10% serum and then incubated for 24 h in serum-free medium, after which the latter culture supernatants were collected and assayed for IL-6 with an ELISA. Data are means±s.d. from three independent experiments. (B) Cells were incubated in medium containing 10% serum for 72 h in the absence or presence of either the BE-8 antibody to IL-6 (1 or 5 μg ml⁻¹) or the JAK inhibitor pyridone 6 (0.5 or 1 μM). Cell lysates were then subjected to immunoblot analysis with antibodies to the indicated proteins.