Table 3.
Substrate Tissue |
d-[U-14C]glucose | d-[U-14C]mannose | l-[1-14C]galactose | |||
Parenchyma | Vascular | Parenchyma | Vascular | Parenchyma | Vascular | |
% Incorporation to AsAa | 1.4 ± 0.06 | 3.1 ± 0.23 | 2.7 ± 0.06 | 5.9 ± 0.23 | 19.9 ± 0.92 | 49.8 ± 0.46 |
Uptake (μCi gFW-1)a | 0.65 ± 0.07 | 3.61 ± 0.05 | 0.32 ± 0.02 | 3.49 ± 0.05 | 0.09 ± 0.01 | 0.67 ± 0.02 |
Uptake (nmol gFW-1)a | 2.1 ± 0.23 | 11.6 ± 0.17 | 1.0 ± 0.06 | 11.4 ± 0.17 | 1.6 ± 0.12 | 12.2 ± 0.23 |
a Values are presented as mean ± S.E., n = 3. Samples were prepared and incubated as described in the text. After incubation samples were extracted with 5% (w/v) PCA containing 5 mM TCEP and AsA purified as described in materials and methods. Radiolabel distribution to AsA was quantified by HPLC using online flow scintillation detection.