Fig. 5.

Reduced cholesterol and triglycerides in Mia2^cpto mice. A: Total cholesterol in FPLC fractions of pooled plasma from Mia2^+/+ (WT, n = 4), Mia2^cpto/+ (HET, n = 6), and Mia2^cpto/cpto (MUT, n = 6) mice. VLDL/LDL and HDL peaks are marked. Cholesterol levels in the MUT pool were lower in fractions 6–24, including VLDL, LDL, and HDL. B: Triglyceride levels in the same FPLC fractions as in part A were again lower in MUT animals. C: Direct measurement of cholesterol (left panel) and triglycerides (right panel) in liver samples from HET and MUT mice revealed no difference in cholesterol levels, but did show a trend toward increased triglyceride levels in MUT livers. Data are expressed as mean ± SEM. D–F: Apolipoproteins in FPLC fractions from part A. D: ApoA-I was modestly lower in HDL fractions from pooled MUT plasma, but not to the same extent as the cholesterol in A. E: Amounts of apoB-100 in MUT VLDL/LDL fractions were not discernibly different from those in HET fractions. F: Although the levels of apoE in MUT fractions are low, they are also highly variable between HET and WT fractions, suggesting that the differences are not significant.