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Immunocytochemistry on HT29. Protein level of CXCR4 in HT29 was characterized by immunocytochemistry using rabbit polyclonal CXCR4 antibody and FITC-conjugated goat-antirabbit IgG. A) Negative control with deletion of the primary antibody. B) HT29 transfected with nontarget siRNA control. C) HT29 transfected with CXCR4 siRNA I and II. D) HT29 transfected with CXCR4 siRNA/ dextran-spermine.
