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Real-time reverse-transcription polymerase chain reaction was performed on a RotorGene 3000 machine, and data analysis of CXCR4 and β-actin expression used the delta-delta CT method. A standard curve was done with a dilution of 1:2. A) CXCR4 expression (standard curve with efficiency 1.34 and an R^2 value of 0.99. E CXCR4 expression in Groups A–E.
