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. 2011 Sep 8;6:1863–1874. doi: 10.2147/IJN.S23949

Figure 3.

Figure 3

Animal survival study. The 4T1 tumor-bearing mice were treated with free MTX, MTX-HSA NPs, biotin 3.66-MTX-HSA NPs, biotin 7.02-MTX-HSA NPs, and biotin 9.41-MTX-HSA NPs. The drugs were injected intravenously in a single dose (day 0). The doses were equivalent to 12.5 mg/kg of free MTX. The curve shows the number of 4T1 tumor-bearing mice still alive on different days.

Abbreviations: MTX, methotrexate; MTX-HSA NP, methotrexate–human serum albumin conjugated nanoparticle; biotin 3.66-MTX-HSA NPs, biotin targeted methotrexate–human serum albumin nanoparticles: 3.66 ± 0.22 biotin/human serum albumin molar ratio; biotin 7.02-MTX-HSA NPs, biotin targeted methotrexate–human serum albumin nanoparticles: 7.01 ± 0.14 biotin/human serum albumin molar ratios; biotin 9.41-MTX-HSA NPs, biotin targeted methotrexate–human serum albumin nanoparticles: 9.41 ± 0.34 biotin/human serum albumin molar ratio.