Table 1.
Physico-chemical characteristics of MTX-HSA NPs (MTX/HSA molar ratio = 8) and biotin functionalized MTX-HSA NPs (mean ± standard deviation; n = 3)
| Biotin/HSA molar ratio | MTX-HSA NPs | Biotin 3.66-MTX-HSA NPs | Biotin 7.01-MTX-HSA NPs | Biotin 9.41-MTX-HSA NPs |
|---|---|---|---|---|
| 0 | 3.66 ± 0.22 | 7.01 ± 0.14 | 9.41 ± 0.34 | |
| Particle size (nm) | 111.70 ± 4.60 | 125.10 ±3.40 | 132.40 ± 2.14 | 144.36 ± 3.80 |
| Poly dispersity index | 0.10 ± 0.01 | 0.21 ± 0.07 | 0.19 ± 0.04 | 0.24 ± 0.05 |
| Zeta potential (mV) | −12.10 ± 0.5 | −20.23 ± 1.11 | −19.56 ± 2.01 | −21.54 ± 1.25 |
Abbreviations: MTX-HSA NPs, methotrexate–human serum albumin conjugated nanoparticles; biotin 3.66-MTX-HSA NPs, biotin targeted methotrexate–human serum albumin nanoparticles: 3.66 ± 0.22 biotin/human serum albumin molar ratio; biotin 7.01-MTX-HSA NPs, biotin targeted methotrexate-human serum albumin nanoparticles: 7.01 ± 0.14 biotin/human serum albumin molar ratio; biotin 9.41-MTX-HSA NPs, biotin targeted methotrexate–human serum albumin nanoparticles: 9.41 ± 0.34 biotin/ human serum albumin molar ratio.