Table 2.

Effect of biotin targeting on the survival of 4T1 tumor-bearing mice

Group	Median survival time (day)	Life span increase (%)
Saline	15.01 ± 0.79	–
MTX	17.51 ± 0.60	16.66
MTX-HSA NPs	22.52 ± 0.50	50.10
Biotin 3.66-MTX-HSA NPs	32.5 ± 0.41	116.66
Biotin 7.01-MTX-HSA NPs	37.5 ± 0.67	150.10
Biotin 9.41-MTX-HSA NPs	47.5 ± 0.71	216.66

^*Note: The dose was equivalent to 6.25 mg/kg of free MTX.

Abbreviations: MTX, methotrexate; MTX-HSA NPs, methotrexate–human serum albumin conjugated nanoparticles; biotin 3.66-MTX-HSA NPs, biotin targeted methotrexate–human serum albumin nanoparticles: 3.66 ± 0.22 biotin/human serum albumin molar ratios; biotin 7.02-MTX-HSA NPs, biotin targeted methotrexate– human serum albumin nanoparticles: 7.01 ± 0.14 biotin/human serum albumin molar ratio; biotin 9.41-MTX-HSA NPs, biotin targeted methotrexate–human serum albumin nanoparticles: 9.41 ± 0.34 biotin/human serum albumin molar ratio.