Figure 4.

Cell-cycle distributions after treatment with either 2ME alone or loaded with MNPs-Fe₃O₄ (100:1) for 24 hours. (A) Control group. (B) MNPs-Fe₃O₄ alone. (C) 1 μM 2ME. (D) 2 μM 2ME. (E) Copolymer of 1 μM 2ME with MNPs-Fe₃O₄ (100:1). (F) Copolymer of 2 μM 2ME with MNPs-Fe₃O₄ (100:1).

Notes: *P > 0.05, when compared with the control group; ^#P < 0.05, when compared with MNPs-Fe₃O₄ and control groups; **P < 0.05, when compared with 2ME alone.

Abbreviations: MNPs-Fe₃O₄, magnetic nanoparticles of Fe₃O₄; 2ME, 2-methoxyestradiol.