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. 2011 Jul 7;286(36):31697–31706. doi: 10.1074/jbc.M111.246843

FIGURE 6.

FIGURE 6.

Antibacterial assay of SLN and PLB. His-tagged proteins were expressed in E. coli. Cell viability was determined by a plating assay of colony formation. Proteins expressed were chloramphenicol transferase (CAT), PLB, SLN, and I17A. Cell viability was determined after protein expression, as normalized to the number of colonies before induction (CON). Immunoblotting using His tag antibody demonstrated similar expression levels for PLB, SLN, and I17A. Error is reported as mean ± S.E. (n ≥ 3). While CON and CAT show statistical equivalence, all other pairwise comparisons are statistically different (p < 0.01).