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The suspension assay. Bacteria were incubated in 1× TSB (3% w/v) with 25 μg/ml CPGRP-S, either alone (△) or supplemented with 100 μg/ml S. aureus MDP (○), 100 μg/ml LPS (□), or no additives (▿). Tubes were shaken at 300 rpm for 5 h, and bacterial density was monitored by measurement of optical density at 600 nm at 1-h intervals. The data represent the mean ± S.D. values of three independent experiments.
