FIGURE 6.

Digoxin inhibits IL-17 production but not IFN-γ production from CD4⁺ T cells derived from EAE mice. CD4⁺ T cells from MOG-immunized mice (Day 9) were stimulated with 1 μg/ml of soluble anti-CD3e Ab and 1 μg/ml of soluble anti-CD28 Ab for 24 h. Cytokine concentrations of the supernatants were determined by HTRF (for IL-17, open circles) and AlphaLISA (for IFN-γ, closed squares). Data were shown as the mean ± S.D. for quadruplicate samples measuring % of DMSO-treated control. **, p < 0.01, ***, p < 0.001 compared with each DMSO-treated control. All experiments were repeated at least once.