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Tryptase and SLIGKV do not influence cell viability or NGF mRNA levels. A, both tryptase and SLIGKV did not alter cellular ATP levels (n = 7; means ± S.E.; RLU, relative luminescence units). B, semiquantitative RT-PCR (n = 5; top) and quantitative real time PCR (n = 4; bottom) showed that levels of NGF mRNA were not significantly affected by treatment with tryptase or the agonist peptide SLIGKV after 24 h. Bars are means + S.E.; n.s., not significant).
